During last night’s broadcast of The Tonight Show with NBC host Jay Leno, comedian Don Rickles made for an unexpectedly hilarious interview with Bristol Palin, daughter of the former Alaska governor.

When Palin admitted to Leno that her mother did not know how she’d lost her virginity — and then described it — Rickles seemed to laugh out loud and wave his hands, making the sign of the cross as if he were horrified.

Then, when Leno asked Palin whether her mother has decided to run for the presidency, Rickles interjected, “She could lose,” drawing a wave of laughter from the crowd.

This video is from NBC’s The Tonight Show, broadcast Thursday, July 14, 2011.