Lab study raises questions over nano-particle impact

PARIS — Tests involving chickens have raised questions about the impact on health from engineered nano-particles, the ultra-fine grains commonly used in drugs and processed foods, scientists said on Sunday.

Chickens exposed to high oral doses of polystyrene particles 50 nanometres (50 billionths of a metre) across absorbed less iron in their diet, according to their study.

At the same time, birds that were chronically exposed to these doses had a "remodelling" of their intestinal villi, the microscopic finger-like projections that play an important role in absorbing nutrients.

The changes meant that the villi increased the surface area available for taking in iron.

Intestinal uptake of calcium, copper, zinc and vitamins A, D, E and K may also be affected by high exposure to nanoparticles, although further research is needed to investigate this, say the authors.

The team, led by Michael Shuler of Cornell University in New York, tested the particles on chickens as a substitute for the human intestine and also used lab-dish cells from the lining of the human gut.

The chickens were given roughly the same dose, weight for weight, as an adult human in a developed country.

"The intestinal epithelial layer represents the initial gate that ingested nanoparticles must pass to reach the body," says the paper, which appears in the specialist journal Nature Nanotechnology.

"The polystyrene particles used in these experiments are generally considered non-toxic, but their interaction with a normal physiological process suggests a potential mechanism for a chronic, harmful, but subtle response."

Engineered nano-particles are used increasingly in the form of titanium oxide or as aluminium silicates in pills to help deliver pills and in food, where they are used as stabilisers or anti-caking agents in fluids and creams.

In developed countries, individuals may be consuming each day a thousand billion engineered particles ranging from fine to ultrafine in scale, according to figures from 2002 research quoted in the study.

Previous research has suggested micron- and nano-sized particles could play a role in the painful inflammatory gut disorder called Crohn's disease, says the paper.

Most of these particles have a negatively-charged surface, which means they adhere to biomolecules in the gut, accumulating at lymphoid nodules called Peyer's patches, according to the earlier research.