Washington (AFP) - US President Joe Biden said Friday he would make it "very, very difficult" for Russia to launch any invasion of Ukraine, which warned that a large-scale attack may be planned for next month.
Washington and Kiev say Moscow has massed troops near Ukraine's borders and accuse Russia of planning an invasion.
Biden and President Vladimir Putin are due to hold a video call shortly to discuss the rising tensions, both sides confirmed Friday.
Biden told reporters in Washington he was putting together "the most comprehensive and meaningful set of initiatives to make it very, very difficult for Mr Putin to go ahead and do what people are worried he may do."
Moscow seized Crimea from Ukraine in 2014 and has since backed separatists fighting Kiev in the east of the country. The conflict has left more than 13,000 dead.
"The most likely time to reach readiness for escalation will be the end of January," Ukrainian Defense Minister Oleksiy Reznikov told parliament in Kiev on Friday.
A report in the Washington Post on Friday citing US officials and an intelligence document said Russia was planning a multi-front offensive involving up to 175,000 troops as soon as next year.
Reznikov said a "winter training period" had started in Russia and that Moscow had already launched exercises near Ukrainian territory.
He estimated that Russia had around 100,000 troops near Ukraine's border. Russia denies any military build-up.
'This is our land'
On the frontline in eastern Ukraine, government troops said they were ready to repel any Russian assault.
"Our task is simple: not to let the enemy enter our country," a soldier named Andriy, 29, told AFP, smoking in a trench near the frontline town of Svitlodarsk.
"All our guys are ready to hold them back. This is our land, we will protect it until the end," another soldier, Yevgen, 24, added.
Kremlin foreign policy adviser Yuri Ushakov told reporters a date had been agreed for the Putin-Biden video summit but would not be announced until final details of the talks have also been set.
US Secretary of State Antony Blinken said the call was expected "in the very near future."
Asked if he had spoken to Putin on Friday morning, Biden shouted "no" as he left a press conference in Washington.
He gave no further details of his planned "initiatives" on Ukraine.
Putin has warned the West and Kiev against crossing the Kremlin's "red lines" over staging military exercises and building up weaponry in Ukraine.
When reporters asked Biden whether he would accept Putin's line, he responded, "I won't accept anybody's red line."
Despite increased contacts between the two rivals since Putin and Biden met for the first time at a summit in Geneva in June, tensions remain high.
As well as the Ukraine conflict, Russia and the United States continue to argue over cyberattacks and the staffing of their embassies, after several waves of diplomatic expulsions.
Ukrainian Foreign Minister Dmytro Kuleba told AFP on Friday that it rejects any efforts to get it to scrap its plans to join NATO.
Moscow wants to see an end to NATO's eastward expansion, after much of Eastern Europe joined the alliance following the collapse of the Soviet Union.
Agreeing to abandon its plans to join the alliance "is not an option," Kuleba said on the sidelines of an OSCE meeting in Stockholm.
"I reject this idea that we have to guarantee anything to Russia. I insist that it's Russia who has to guarantee that it will not continue its aggression against any country," he said.
NATO officially opened the door to Ukraine membership in 2008, though no progress has been made since.